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12-15 August 2018
Europe/Berlin timezone

How thick is your light sheet? A guide to quantitative measures

Not scheduled


Pfotenhauerstraße 108 01307 Dresden Germany
Poster Posters


Ms Elena Remacha (IGBMC, France; Leica Microsystems GmbH, Germany)


Several novel Light Sheet Fluorescence Microscopy techniques have emerged in recent years in pursue of good axial resolution over a long field of view. These include Bessel, Lattice and Airy beam light-sheet microscopy, among others. There has not been a direct comparison in the literature of their dimensions, and often different criteria are used among publications. Most of them present complex geometries that, in addition, are rapidly changing along the propagation direction, which makes the measurement troublesome. Here we propose a unified criterion to measure the length and thickness for all the currently available variants of light sheet. We have applied this methodology to our results of numerical simulations of the propagation of beams with the geometries stated above. Considering gaussian beams as the basis, we offer quantitative measurements and a direct comparison of the beams considered. With this we aim at providing a guideline for the scientific community on the choice of the most appropriate technique for their specific application.

Affiliation IGBMC, France; Leica Microsystems GmbH, Germany
Terms and Conditions Yes

Primary authors

Ms Elena Remacha (IGBMC, France; Leica Microsystems GmbH, Germany) Dr Julien Vermot (IGMBC, France) Dr Florian O. Fahrbach (Leica Microsystems GmbH, Germany)

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