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12-15 August 2018
Europe/Berlin timezone

Light Sheet Illumination can enhance the resolution of Light Field microscopy

Not scheduled


Pfotenhauerstraße 108 01307 Dresden Germany
Poster Posters


Manu Forero-Shelton (Department of Physics, Universidad de los Andes, Colombia)


Light sheet fluorescence microscopes have high spatial resolution but sequential scanning of the planes limits the maximum imaging speed that can be achieved. On the other hand, light field microscopes and other extended depth of field imaging methods have high temporal resolution but suffer from low spatial resolution. Here we show a method to dynamically increase the spatial resolution of a light field microscope using multiple light sheet illumination. This method essentially allows trading the temporal resolution of the light field for increased spatial resolution, with the possibility of making this tradeoff on the fly with software. This approach extends the versatility and the range of applicability of light field microscopy, without additional hardware changes, enabling fast adaptation of the microscope to the requirements of different biological experiments.

Affiliation Universidad de los Andes, Bogota, Colombia
Terms and Conditions Yes

Primary author

Jorge Madrid-Wolff (Department of Biomedical Engineering, Universidad de los Andes, Colombia)


Mr Diego Castro (Department of Physics, Universidad de los Andes, Colombia) Mr Nicolas Barbosa-Berrio (Department of Physics, Universidad de los Andes, Colombia) Prof. Omar E. Olarte (Vicerrectoría de Investigación, Universidad ECCI) Manu Forero-Shelton (Department of Physics, Universidad de los Andes, Colombia)

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