10th Anniversary Light Sheet Fluorescence Microscopy Conference

Inverted Lattice-Light Sheet Microscope for long-term imaging on embryonic development

Not scheduled

15m

MPI-CBG

Poster Posters

Speaker

Dr Li-Ling Yang (European Molecular Biology Laboratory)

Description

Lattice light-sheet microscopy [1] provides fast volumetric images with subcellular resolution from cells to embryos. To expand its capacity to a wider range of biological applications, we implemented an inverted version of a lattice light-sheet microscope [2]. The inverted lattice light-sheet microscope adopts a two chamber-design, which separates the biological samples from the immersion liquid of the two objectives. The open-top design further allows for atmospheric control (N2, O2, CO2 and humidity) besides the temperature to support the long-time embryonic development over days, and suppress the photobleaching effect. Two different sample mounting schemes are available for iLLS: a) an adaptor to hold the coverslip with samples attached upside down and dipped into the isolation chamber, and b) a sample carrier made from a 50m/25m thick FEP folio carrying larger samples such as embryos or cultured cells. We demonstrate live images of Drosaphila embryos, mouse embryos and cultured cells. iLLS together with DNA-PAINT (point accumulation for imaging in nanoscale topography) also to super-resolve tubulin structure inside cultured cells is also shown.

[1] Science, vol.346, 1257998-1, 2014
[2] Nat. Meth., vol. 13, 139, 2016