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12-15 August 2018
MPI-CBG
Europe/Berlin timezone

Advanced Light sheet Imaging Center (ALICE) Development of a full-service imaging platform – from sample clarification to 3D VR visualization

Not scheduled
15m
MPI-CBG

MPI-CBG

Pfotenhauerstraße 108 01307 Dresden Germany
Poster Posters

Speaker

Stephane Pages (Wyys Center for Bio and Neuro Engeeniring)

Description

Lightsheet microscopy is a fluorescence imaging technique that allows visualization of whole organs or small organisms while preserving their physical integrity i.e. without the need to slice them prior imaging. Although the principle of operation of this technology was developed more than 100 years ago, it is only in the last fifteen years that biologists have commonly started to use such microscopes. Since that time, this type of microscopy has become a standalone field of research that has never been as active as it is now. At the Wyss Center for Bio and Neuroengineering in Geneva, Switzerland, we have created an imaging center, available to everyone, which integrates a series of cutting edge and custom-tailored tools into a single working pipeline aimed at imaging whole organs at high temporal or spatial resolution. We then analyze the data in a Virtual Reality environment. The center includes a customized version of the COLM/SPED (originally designed by Prof. Raju Tomer - Columbia University) microscope for near diffraction-limited resolution imaging of large clarified samples (cm range). Recently we expanded the capabilities of lightsheet microscopy, setting up a large-scale imaging system: mesoSPIM (see poster from Fabian F. Voigt). This customized system enables whole brain imaging at cellular resolution, in a few minutes with no need for further stitching processes. Finally, we are developing innovative 3D exploration and analysis tools that will enable researchers to navigate and segment their own lightsheet data in a virtual reality environment. This complete pipeline offers to the researcher the possibility for large scale screening, high resolution imaging and data visualization and analysis.

Terms and Conditions Yes
Affiliation Wyys Center for Bio and Neuro Engeeniring

Primary author

Stephane Pages (Wyys Center for Bio and Neuro Engeeniring)

Co-authors

F. Fabian Voigt (Brain Research Institute, University of Zurich) Laura Batti (Wyss Center, Geneva) Dr Gilles Reymond (Wyys Center for Bio and Neuro Engeeniring) Dr Ronan Chereau (CMU-UNIGE) Dr Quentin Barraud (EPFL) Mr Newton Cho (Center for Neuroprosthetics and Brain Mind Institute, School of Life Sciences, Swiss Federal Institute of Technology (EPFL), ) Mr Jordan Squair (Center for Neuroprosthetics and Brain Mind Institute, School of Life Sciences, Swiss Federal Institute of Technology (EPFL), ) Dr Corinne Brana (Wyss Center for Bio and Neuroengineering) Mrs Audrey Tissot (Wyss Center for Bio and Neuroengineering) Mr Fabien Moreillon (University of Applied Sciences and Arts Western Switzerland (HES-SO), Geneva, Switzerland) Prof. Philippe Passeraub (University of Applied Sciences and Arts Western Switzerland (HES-SO), Geneva, Switzerland) Prof. Grégoire Courtine (Center for Neuroprosthetics and Brain Mind Institute, School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Geneva, Switzerland.) Fritjof Helmchen (Brain Research Institute, University of Zurich) Prof. Michael Zeneih (Stanford University, Stanford, CA, USA) Prof. Maged Goubran (Stanford University, Stanford, CA, USA) Prof. Karl Deisseroth (Stanford University, Stanford, CA, USA) Prof. Tomer Raju (Columbia University, NY, NY, USA) Anthony Holtmaat (University of Geneva) Christian Lüscher (University of Geneva) Prof. John Donoghue (Wyss Center for Bio and Neuroengineering, Geneva, Switzerland)

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