Organoid cultures have been recently established to study organ formation and tissue morphogenesis. However, imaging of these samples has been hampered by their long and often inefficient development and their light sensitivity. Light sheet microscopy would be the imaging technique of choice due to its low photo-toxicity and high acquisition speed. However, many current light sheet microscopes...
Tissue clearing methods have recently seen a renaissance with a wide variety of clearing approaches now available. In neuroscience, the combination of tissue clearing with light-sheet microscopy is ideal to bridge scales from the µm to cm-level, thus providing a link on the mesoscale for detailed 3D anatomical investigations. To optimally image cleared samples, we set out to design a modular...
The use of exotic optical modes is becoming increasingly widespread in microscopy. Particularly, propagation-invariant beams, such as Airy and Bessel beams and optical lattices, have been particularly useful in light-sheet fluorescence microscopy (LSFM) as they enable high-resolution imaging over a large field-of-view (FOV), possess a resistance to the deleterious effects of specimen induced...
