We were able to generate extremely long thin sheets of light in the one micron range and a vastly increased Rayleigh length by breaking the diffraction limit of light sheets of low numerical aperture. We measured the thickness of the light sheets with different methods including standard point spread function measurement with fluorescent beads. By using these light sheets in our...
Throughout the last decades, access to genetic perturbations, fluorescent labels and modern microscopy advanced our molecular understanding of cell-biological processes tremendously.
The spatio-temporal organization of cells and developing embryos that we observe under these microscopes is widely believed to depend on physical processes such as diffusion and motor-driven intracellular flows....
Light Sheet Microscopy made great progress during last 15 years. We can now see its transition from the hands of developers to the hands of ''ordinary'' scientists who work in the field of biomedical research. Core imaging facilities can, in our opinion, play great role in supporting such transition. In our talk we will share the lessons which we learned during last 12 years when we have been...
