Light sheet microscopy allows live 3D imaging of entire developing embryos with
high spatial and temporal resolution. Computational analysis of these recordings
promises new insights in developmental biology. However, a single dataset often
comprises many terabytes, which makes storage, processing, and visualization of
the data a challenging problem. The open-source Fiji platform provides...
During development coordinated cell behaviors orchestrate tissue and organ morphogenesis to suit the lifestyle of the organism. We have used here the crustacean Parhyale hawaiensis to study the cellular basis of limb development. Transgenic Parhyale embryos with fluorescently labeled nuclei were imaged at high spatiotemporal resolution using multi-view light-sheet fluorescence microscopy...
To quantitatively understand biological processes that occur over long time periods, it is desirable to image multiple samples simultaneously, and automatically process and analyze the resulting datasets. Here, we present a comprehensive and dedicated multi-sample image acquisition and processing workflow using selective plane illumination microscopy (SPIM) to image several embryos up to 4...
