Most biological processes involve spatial-temporal changes in the concentration of proteins that ensure that the right protein acts at the right place at the right time. Due to its high temporal resolution and minimal photo bleaching light sheet microscopy is ideally suited to visualize such protein dynamics given that the protein of interest is labelled with a fluorescent probe. Indeed,...
Our digestive tracts are home to trillions of microbes that immigrate, emigrate, reproduce, and compete with one another. Little is known about the physical structure and temporal dynamics of gut microbial communities, which must necessarily influence the function not only of normal, commensal communities but also community invasion by pathogens. To address this, my lab applies light sheet...
Three-dimensional molecular mapping of RNA expression within intact biological tissue is allowing for new insight into the relationship structure and function. Current RNA quantification approaches are depth-limited to less than 200 $\mu m$ by the requirement for single-molecule read out of sequential barcoded RNA fluorescence in-situ hybridization (RNA-FISH) or hydrogel embedded in-situ RNA...
