For many developing tissues, their shape is established early in development. In order to maintain this shape during subsequent growth, these tissues need to scale isotropically. The way by which cells inside tissues enable coordinated, isotropic tissue scaling is not understood, however, as most studies focused on changing, rather than maintaining tissue shapes during development. In this...
Understanding the brain requires measuring and perturbing neuronal activity. Tools for this are typically applied locally, but behavior is generated by the coordinated activity of neurons widely distributed across the brain. Thus, ideally we want to measure activity patterns of all neurons in the brain during behavior, use this information to decide which neurons to perturb, and record the...
Drug screens on complex cell models and organisms are a key factor to understand and treat human diseases. However, fast and effective conclusions have been hindered by the lack of robust and predictable models amenable to high-throughput (HT) analysis. Recently, important advances have been made towards the development of 3D co-culture models using distinct cell types that better...
Although the low-impact nature of light sheet microscopy has opened up new avenues for developmental timelapse imaging, the heart remains a particularly challenging organ to image in 3D timelapse. To image processes on timescales of minutes to hours (such as heart development, cell migration, repair and regeneration) demands some form of synchronized image acquisition in order to separate the...
Light sheet microscopy is an ideal tool for precision mapping of flow fields on a microscopic scale, due to its optical similarities with particle image velocimetry, which on a macroscopic scale is a mature technique in fields such as aerospace engineering. Several challenges exist that mean it is difficult to obtain high-quality 3D velocity data in a living sample, but we will show how...
